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Sangon Biotech tbe urea sample buffer
Tbe Urea Sample Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbe+urea+sample+buffer/buffer+tbe/pm42098583-34-14-28
Average 86 stars, based on 1 article reviews
tbe urea sample buffer - by Bioz Stars, 2026-09
86/100 stars

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Article Title: A well-designed bridge probe with dual functions of recognition and transduction towards a universal LAMP system.
Article Snippet: in molecular diagnostics [12, 13].. Among the above isothermal amplification methods, LAMP requires only DNA polymerase with strand-substitution activity and constant temperature (65 ± 5 °C) to achieve 109–1010-fold amplification of double-stranded DNA within 15 min to 2 h [14–16].. Owing to its high amplification efficiency, operational simplicity, and tolerance to crude samples, LAMP has been widely adopted in nucleic acid detection and molecular diagnostics.



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(a) IGV screenshots of m 1 A 58 mismatch detected on Pre-tRNA-iMet-CAT-1-1, Pre-tRNA-Met-CAT-1-1 and Pre-tRNA-Ile-TAT-2-3. WT HEK293T total RNA was treated with RppH to increase capture of premature tRNA sequences. Green shade = leader sequences, Green shade = trailer sequences, Orange shade = intron sequences. (b) Number of significantly up- or down-regulated tRNA-derived fragments (p.adj < 0.05 by DESeq2). While tRF-1s are decreased by TRMT6/61A loss, other tRF types are up-regulated. (c) Schematic representation of tRF-1 generation by RNase Z (ELAC2) cleavage of the trailer sequence. (d) Scheme of in vitro RNase Z (ELAC2) assay with and without TRMT6/61A. (e) In vitro RNase Z assay product was resolved on <t>TBE</t> <t>Urea</t> gel to separate the substrate (top band) and the cleaved product (bottom band). (f) Quantification of in vitro RNase Z assay (n = 3, comparison done by paired two-tail t test). See also Supplementary Figure 5 .
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(a) IGV screenshots of m 1 A 58 mismatch detected on Pre-tRNA-iMet-CAT-1-1, Pre-tRNA-Met-CAT-1-1 and Pre-tRNA-Ile-TAT-2-3. WT HEK293T total RNA was treated with RppH to increase capture of premature tRNA sequences. Green shade = leader sequences, Green shade = trailer sequences, Orange shade = intron sequences. (b) Number of significantly up- or down-regulated tRNA-derived fragments (p.adj < 0.05 by DESeq2). While tRF-1s are decreased by TRMT6/61A loss, other tRF types are up-regulated. (c) Schematic representation of tRF-1 generation by RNase Z (ELAC2) cleavage of the trailer sequence. (d) Scheme of in vitro RNase Z (ELAC2) assay with and without TRMT6/61A. (e) In vitro RNase Z assay product was resolved on <t>TBE</t> <t>Urea</t> gel to separate the substrate (top band) and the cleaved product (bottom band). (f) Quantification of in vitro RNase Z assay (n = 3, comparison done by paired two-tail t test). See also Supplementary Figure 5 .
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(a) IGV screenshots of m 1 A 58 mismatch detected on Pre-tRNA-iMet-CAT-1-1, Pre-tRNA-Met-CAT-1-1 and Pre-tRNA-Ile-TAT-2-3. WT HEK293T total RNA was treated with RppH to increase capture of premature tRNA sequences. Green shade = leader sequences, Green shade = trailer sequences, Orange shade = intron sequences. (b) Number of significantly up- or down-regulated tRNA-derived fragments (p.adj < 0.05 by DESeq2). While tRF-1s are decreased by TRMT6/61A loss, other tRF types are up-regulated. (c) Schematic representation of tRF-1 generation by RNase Z (ELAC2) cleavage of the trailer sequence. (d) Scheme of in vitro RNase Z (ELAC2) assay with and without TRMT6/61A. (e) In vitro RNase Z assay product was resolved on <t>TBE</t> <t>Urea</t> gel to separate the substrate (top band) and the cleaved product (bottom band). (f) Quantification of in vitro RNase Z assay (n = 3, comparison done by paired two-tail t test). See also Supplementary Figure 5 .
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(a) IGV screenshots of m 1 A 58 mismatch detected on Pre-tRNA-iMet-CAT-1-1, Pre-tRNA-Met-CAT-1-1 and Pre-tRNA-Ile-TAT-2-3. WT HEK293T total RNA was treated with RppH to increase capture of premature tRNA sequences. Green shade = leader sequences, Green shade = trailer sequences, Orange shade = intron sequences. (b) Number of significantly up- or down-regulated tRNA-derived fragments (p.adj < 0.05 by DESeq2). While tRF-1s are decreased by TRMT6/61A loss, other tRF types are up-regulated. (c) Schematic representation of tRF-1 generation by RNase Z (ELAC2) cleavage of the trailer sequence. (d) Scheme of in vitro RNase Z (ELAC2) assay with and without TRMT6/61A. (e) In vitro RNase Z assay product was resolved on <t>TBE</t> <t>Urea</t> gel to separate the substrate (top band) and the cleaved product (bottom band). (f) Quantification of in vitro RNase Z assay (n = 3, comparison done by paired two-tail t test). See also Supplementary Figure 5 .
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(a) IGV screenshots of m 1 A 58 mismatch detected on Pre-tRNA-iMet-CAT-1-1, Pre-tRNA-Met-CAT-1-1 and Pre-tRNA-Ile-TAT-2-3. WT HEK293T total RNA was treated with RppH to increase capture of premature tRNA sequences. Green shade = leader sequences, Green shade = trailer sequences, Orange shade = intron sequences. (b) Number of significantly up- or down-regulated tRNA-derived fragments (p.adj < 0.05 by DESeq2). While tRF-1s are decreased by TRMT6/61A loss, other tRF types are up-regulated. (c) Schematic representation of tRF-1 generation by RNase Z (ELAC2) cleavage of the trailer sequence. (d) Scheme of in vitro RNase Z (ELAC2) assay with and without TRMT6/61A. (e) In vitro RNase Z assay product was resolved on TBE Urea gel to separate the substrate (top band) and the cleaved product (bottom band). (f) Quantification of in vitro RNase Z assay (n = 3, comparison done by paired two-tail t test). See also Supplementary Figure 5 .

Journal: bioRxiv

Article Title: TRMT6/61A-mediated m 1 A methylation facilitates human pre-tRNA maturation and prevents surveillance by XRN2

doi: 10.64898/2026.05.17.725798

Figure Lengend Snippet: (a) IGV screenshots of m 1 A 58 mismatch detected on Pre-tRNA-iMet-CAT-1-1, Pre-tRNA-Met-CAT-1-1 and Pre-tRNA-Ile-TAT-2-3. WT HEK293T total RNA was treated with RppH to increase capture of premature tRNA sequences. Green shade = leader sequences, Green shade = trailer sequences, Orange shade = intron sequences. (b) Number of significantly up- or down-regulated tRNA-derived fragments (p.adj < 0.05 by DESeq2). While tRF-1s are decreased by TRMT6/61A loss, other tRF types are up-regulated. (c) Schematic representation of tRF-1 generation by RNase Z (ELAC2) cleavage of the trailer sequence. (d) Scheme of in vitro RNase Z (ELAC2) assay with and without TRMT6/61A. (e) In vitro RNase Z assay product was resolved on TBE Urea gel to separate the substrate (top band) and the cleaved product (bottom band). (f) Quantification of in vitro RNase Z assay (n = 3, comparison done by paired two-tail t test). See also Supplementary Figure 5 .

Article Snippet: The reactions were stopped by adding 10 μL of Novex TBE-Urea Sample Buffer (2X) (Invitrogen, #LC6876), and the reactions were run on a 10% Tris-Borate-EDTA (TBE) Urea gel (Invitrogen, #EC68752).

Techniques: Derivative Assay, Sequencing, In Vitro, Comparison